![]() ![]() ![]() However, current species definitions facilitate species-phenotype incongruences, particularly when horizontally acquired genes are responsible for a phenotype. Differentiating organisms capable of causing illness or death from those used in industry is essential for risk assessment and outbreak preparedness. ![]() The Bacillus cereus group comprises numerous closely related species, including bioterrorism agent B. Because distribution breakpoints and shape were robust to the exclusion of genomes at all tested thresholds, the six histograms shown represent pairwise ANI values calculated between raw genomes downloaded directly from NCBI’s RefSeq database (i.e., the least stringent quality thresholds, with no filtering criteria employed n = 2,231 genomes) (A), genomes with N 50 of >20 kb (the set of genomes from which medoid genomes were selected n = 2,218) (B), genomes with N 50 of >0 and no contigs assigned to a domain other than Bacteria ( n = 1,291) (C), genomes with N 50 of >20 kb and no contigs assigned to a domain other than Bacteria ( n = 1,289) (D), genomes with N 50 of >0 and no contigs assigned to a genus outside Bacillus ( n = 1,074) (E), and genomes with N 50 of >100 kb and no contigs assigned to a genus outside Bacillus (i.e., the most stringent quality thresholds tested n = 998) (F). cereus group genomes meeting various quality thresholds. Sample of histograms constructed using pairwise ANI values calculated between B. ![]()
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